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Among the glycopeptides that bound with medium affinity to Aq (20 55% inhibition), two subgroups could be distinguished with respect to their T-cell responses.
A common feature of the glycopeptides that bound with medium affinity to Aq, but induced weak or non-existent T-cell responses, was the presence of a large side chain in p263, i.e. cyclohexylalanine in 6 and 9 and m-methylphenylalanine in 8, 11, and 15.
Similar(58)
Thus, in this in vitro system, both peptides bind with medium affinity (µM range) to HSC70.
The obtained affinity constants indicated that the investigated McAb was an antibody with medium affinity.
The labeled belatacept bound with high affinity to Raji cells.
All Envs bound to CD4 binding site, membrane-proximal, and V1/V2 MAbs with similar apparent affinities, although the T/F Envs bound with higher affinity to the MAb 17b, a CCR5 coreceptor binding site antibody.
The TIR1 binding pocket had the highest affinity for IAA, and although 2,4-D could bind to TIR1, it was bound with weaker affinity [64].
The applied growth conditions in complex rich medium might not be suitable for the detection of all PdhR-DNA interactions, although the overproduction of PdhRhis should facilitate binding, also to DNA fragments which might be bound with low affinity.
The peptides 60029 and 60030 bound with high affinity, whereas the peptide 60031 displayed a weak affinity (Table 1).
We previously demonstrated that SC18 HA bound with high specificity and high affinity to human receptors, while AV18 HA bound with high affinity to avian receptors.
All three peptides bound with high micromolar affinities.
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