GT-4 was found to specifically bind to this sequence from Cor15A promoter.
Based on the currently available ChIP-seq data however, there is currently no evidence that these transcription factors bind to this sequence in vivo.
With the DRA Y-box sequence as probe (Fig. 5b, lanes 6 10), the unlabelled probe sequence competes less well than do any of the other Y-box sequences, suggesting that rDEK alone binds to this sequence nonspecifically and with low affinity.
We have previously shown that a single seven base-pair DNA motif is found in the vast majority of patterning enhancers active in the cellular blastoderm (Li et al., 2008), and that the maternally deposited transcription factor Zelda (ZLD) (Liang et al., 2008), which binds to this sequence, is present at these enhancers by mitotic cycle 8, in the early phase of the MZT (Harrison et al., 2011).
To directly test whether MeCP2 bound to this EGR2 regulatory sequence in neuronal cells, chromatin immunoprecipitation (ChIP) with MeCP2-specific antibodies was conducted on chromatin from 48 h PMA phorbol ester -stimulated SH-SH5Y nester -stimulateds, a system previously demoneuroblastomahow incellsed MeCP2 levels (42).
CBF1-3 proteins bind to this DNA regulatory sequence.
One scenario is that σA binds to this sequence, which is facilitated by the interaction between σ A and αCTD bound to the site upstream of −35.
The recombinant HA-tagged Ets domain of Elk-1 did not bind to this sequence (Fig. 4B, lane 4).
To detect whether OsNAP can bind to this sequence, the ORF of OsNAP was fused to GAL4-AD in the pGADT7 vector and co-transformed with the pHIS2- cis reporter vector [ 34] into the Y187 yeast strain.
Later on, it was found that in the germ line the Specific Alu Binding Protein (SABP) can bind to this sequence and prevent methylation [ 35], but since the importance of this function of is still not understood, and hence the high level of conservation is still a mystery.
