Sentence examples for bound to the template from inspiring English sources
These interactions may also serve as a wedge to displace a complementary oligonucleotide fragment bound to the template in front of the growing DNA chain that may impede chain elongation.
This short inserted sequence in general is not expected to base pair to the template strand, but rather loops out when the primer is bound to the template.
Similarly, as a control reaction, the single-stranded 120 bp DNA was amplified by conventional PCR in which the first primer (PCR-1) bound to the template DNA in an antiparallel orientation and the second primer (PCR-2) annealed to the newly synthesized DNA in an antiparallel orientation.
Even if it turns out that any polymerase ribozyme has to be very long, the preliminary replicase ribozyme may have only been a ligase – short enough and loosely bound to the template-substrate complex – as demonstrated in our computer simulation work concerning the replicase [ 18] (actually just being the Rep in the current model).
The primer annealing temperature determines that the primer completely binds to the template or partially mismatches the template in one or several bases.
Conventionally, in a polymerase chain reaction (PCR), oligonucleotide primers bind to the template DNA in an antiparallel complementary way and the template DNA is amplified as it is.
No ligands are bound to the Upf1 template structure (1PJR).
Six different annealing temperatures were designed according to the Tm of the primers bound to the WT template.
We observed that GST-Arx bound to the Lmo1 template (Fig. 9E, lane 2), and this binding was attenuated by competition with a 50-fold molar excess of unlabeled template (Fig. 9E, lane 3).
HRG was taken from PDB entry 1HAE (Jacobsen et al., 1996) and aligned with the EGF bound to the EGFR template, using EGF's residues 26 46 for reference (as this part appears to be most similar structurally between the two).
Secondly, RFs have to negotiate obstructions arising from proteins tightly bound to the DNA template, DNA secondary structures, ongoing transcription in their path, and buildup of torsional stress in the DNA (Aguilera and Gómez-González 2008).
