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Anti-antibody γ-H2AX bound to the sections was incubated with Vectastain avidin-biotin peroxidase complex (ABC) reagent (Vector Laboratories, UK) for 30 min at room temperature.
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Probe bound to the section was detected using anti-digoxigenin Fab conjugated with alkaline phosphatase and NBT/BCIP as chromogenic substrates (Roche).
When 75 nM cofilin was added to actin filaments immobilized on the lipid surface, cofilin gradually bound to the actin filaments, and sections of filaments bound with cofilin molecules were easily identified, as the bound filaments appeared thicker and the peaks were taller.
Target cells were excised, with at least 100 cells per section, and bound to the transfer film.
Continue adding fasteners until the entire section of hair is bound to the tip.
The sensors, which currently can only be used in animals, include a section that binds to the target molecule, as well as a magnetic component that allows them to become visible with MRI.
Our findings cannot be explained by place cells responding purely to local features (such as junctions) or measuring distances from those features, since the place fields showed no tendency to be bound to specific track sections on the protrusion.
These results suggest that a single cofilin molecule cannot induce conformational changes, either symmetric or asymmetric, that involve detectable changes in helical pitch when it binds to bare sections of the filament.
More box-office records are bound to follow.In this section On the front line Not fade away Blood-stained rainbow Engrossed Deprive and rule New light in Myanmar?
Fluorescence immunohistochemical studies show that the polyclonal antibodies bind to spheroid sections and tumour sections in patterns similar to those revealed by autoradiographic studies with a tritium-labelled derivative of the fluorinated misonidazole analogue.
The experiments show that the loop specifically binds to a section of substrate proteins called the transmembrane segment that spans the cell membrane.
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