Sentence examples for bound to the probe from inspiring English sources

When the two fluorescent dyes bound to the probe are in close proximity (when the probe is intact), the interaction of the two dyes prevents the reporter dye from fluorescing.

ANCHORSMAP employs a new function for ΔG calculations, which is specifically designed for the context of protein-protein recognition by introducing a correction in the electrostatic energy term that compensates for the dielectric shielding exerted by a hypothetical protein bound to the probe.

Then the mixture was subjected to SDS-PAGE and the proteins bound to the probe were visualized by Western blotting using anti-FLAG antibody M2 (Sigma-Aldrich, MO).

In addition, a slightly retarded band was observed when the as-2 [25] probe (−107 to −83) was used with gentian nuclear extract, whereas tobacco nuclear extract strongly bound to the probe.

Only the protein(s) bound to the probe is (are) radioactively labelled.

EMSA revealed that purified AtVOZ2 bound to the probe in a dose-dependent manner.

In the experiments described here, the chosen double-stranded DNA dye (SYBR Gold I) was excited by excitation light source of the ABI 7700 at 480 nm when bound to the probe-target hybrid and served, in turn to excite the Cy5 fluorophore on the probe.

It involves a selection of specific genomic loci through adhesion to probes and sequencing only the DNA fragments that are bound to the probes.

Equilibrium is usually preferred for hybridization because the signal strength is greatest, since the maximum numbers of target molecules are bound to the probes.

Fur bound to the probes corresponding to the promoter region of CC2193 (data not shown,[ 6]) as well as to that of CC3059, validating the Fur binding sites found overlapping the −35 and −10 promoter elements of these two genes.

However, as both target and probe are present in a large number of copies, according to the law of probability, nearly half of the target oligonucleotides bind to the probe in the downstream.