Sentence examples for bound to the primary from inspiring English sources
After incubation and washing, strong magnets were used to remove all beads that bound to the primary or secondary antibody.
The secondary antibody Goat Anti-Mouse IgG AP conjugate bound to the primary antibody (IgG) and the signal was enhanced.
The protein bands specifically bound to the primary antibodies were detected using an anti-rabbit IgG alkaline phosphatase-linked secondary antibody and an ECF (enhanced chemifluorescence) Western blot analysis system (Amersham Pharmacia Biotech, Piscataway, NJ, USA) (Ouyang et al. 2006).
The protein band, specifically bound to the primary antibody, was detected using an anti-rabbit IgG-alkaline phosphatase (AP)–linked antibody and an electrochemifluorescence (ECF) Western blotting system (Amersham Biosciences, Piscataway, NJ, USA).
The protein band, specifically bound to the primary antibody, was detected using an anti-rabbit IgG-alkaline phosphatase-linked antibody and an enhanced chemifluorescence Western blotting system (Amersham Biosciences, Piscataway, NJ).
DNase I footprinting analysis of DevRC with the tgs1-Rv3131 interegion revealedevealed that it binds to the primary site, but fails to cooperatively bind to the adjacent site, unlike full-length DevR which protected both the sites (Fig. 2).
Thus, it could be deduced that the reduction of Au3+ by APM occurred via the COOH group, and the primary NH2 bound to the surface of Au nanoparticles.
Following another three-step washing cycle as mentioned earlier with the buffer, the presence of the primary antibody bound to the tissue antigen was detected using liquid-stable 3,3´-diaminodbenzidine tetrahydrochloride (DAB, Sigma) solution as a dye substrate for alkaline phosphatase.
Horseradish peroxidase-coupled anti-mouse IgG secondary antibody (diluted 1 1,000 in 5% milk + PBS-Tween and applied for 1 h at room temperature) was used to detect primary antibodies bound to the blot, together with enhanced chemiluminescence reagent (GE Healthcare).
