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The mathematical model presented takes into account the viscoelastic properties of the polymer and the state of the dispersed drug: free or chemically bound to the matrix.
TGF-β1 was both conjugated through the PEG linker and bound to the matrix via physical affinity, delaying the release rate of TGF-β1 up to 10 days.
Tagged proteins bound to the matrix were released by boiling in electrophoresis sample buffer.
Under these conditions, the Oto protein elutes in a distinct peak from Oto-modified Wnt3a (not shown), indicating that Oto and Wnt3a bound to the matrix independently.
RNA is subsequently bound to the matrix of the column, washed to remove contaminants and then eluted with molecular biology grade water.
TIMP-1, TIMP-2 and TIMP-4 are present in soluble forms, while TIMP-3 is tightly bound to the matrix [ 4].
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ECs can also bind to the matrix through the binding of Ang-1 to integrins, which can mediate some of the effects of Ang-1 without Tie2 phosphorylation [ 146, 151].
In contrast, the bound phenolic compounds, which are covalently bound to the plant matrix, cannot be extracted into water or aqueous/organic solvents mixtures [ 7].
Scanning electron microscopy and fluorescent microscopy confirmed that bacterial cells were trapped within the GO filter matrix and binding strength tests showed that cells were strongly bound to the GO matrix.
DNA not bound to the nuclear matrix is eluted in a NaCl gradient (DNA 0), DNA "loosely" bound to the nuclear matrix is released using a LiCl-urea gradient (DNA I), high temperature is necessary to release DNA from the strong complexes with the nuclear matrix (DNA II).
Once the phosphopeptides or phosphoproteins are bound to the chromatography matrix, it is of key importance to elute the vast majority of bound phosphopeptides/proteins (ideally all of them) out of the matrix.
More suggestions(17)
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bind to the matrix
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bound to the corpse
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bound to the promoter
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bound to the time
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