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In summary, nvp63 bound to the consensus DNA sequence recognized by hup53 and transactivated target gene transcription in vitro.
Like vertebrate estrogen receptors, the mollusc ER protein bound to the consensus vertebrate estrogen-response element (ERE).
Protein bound to the consensus sequence was then treated with specific primary antibody, which was then be detected using HRP labeled secondary antibody and a colourimetric substrate.
A colorimetric p65 transcription factor assay was used to assess the amount of nuclear p65 bound to the consensus NF-κB response element immobilized on the assay plate.
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Under certain conditions, NF-κB can also bind to the consensus sequence of the Sp1 binding site, thereby competing with Sp1 [61], [61].
They recognize and bind to the consensus DNA sequence CTA(A/T 4TAG/A as homo- or heterodimers via a 56-amino acid domain (i.e. MADS-box) [7], [8].
GATA3 is a dual zinc finger transcription factor that binds to the consensus motif (A/T GATA(A/G) on the target gene promoter region [ 5– 7].
The AhR is activated by binding to ligands, including dioxins, translocates from the cytoplasm to the nucleus and then binds to the consensus sequence known as XRE (xenobiotic responsive element) [ 7].
Since the largest portion of the activation effect occurred between −474 and −823 bp of the promoter (Fig. 2C), we evaluated the DNA binding activities of proteins bound to the putative consensus sequences within this region using gel shift analyses.
Furthermore, atRA decreased the intensity of TNF-α-activated TNF-α-activated TNF-α-activatednsus site.
We then asked whether these mCpG-binding TFs also bound to the same consensus motifs in an unmethylated form.
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