Sentence examples for bound to the column from inspiring English sources

M, protein marker; hLF, human lactoferrin standard; TG, milk samples from transgenic cows; P1 and P2, rhLF eluted from the column; FT, protein fraction that was not bound to the column.

SCARB2 bound to the column was eluted in a buffer containing 50 mmol/L imidazole.

MtbHadAB complex bound to the column was eluted with a 0.05 1 mol/L linear gradient of NaCl.

Proteins bound to the column were then eluted with elution buffer (column buffer with 1 M NaCl).

Washing the protein bound to the column with buffer (10 mM Tris HCl, 500 mM NaCl; pH 7.5) completely removed the weakly bound FAD.

Proteins bound to the column were then eluted with elution buffer (column buffer with 10 mM maltose) at a flow rate of 0.5 ml/min.

Antibodies recognizing the phospho-residue bound to the column which was eluted as phospho-specific antibodies.

Proteins associated with RESCUE activity bound to the column and were eluted at low salt concentrations (Figure S2).

The proteins bound to the columns were eluted using 500 mM NaCl and fractions were collected.

The proteins bound to the columns were eluted and concentrated on microfilterfuge tubes with a 10 kD cut off.

Two proteins that specifically bound to the columns were of interest: LRP1 (low-density lipoprotein receptor-related protein 1) (Grey et al., 2004) and MRC2 (mannose receptor C, type 2) (Engelholm et al., 2009).