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Signal intensities were normalized with the total amount of radioactivity bound to the arrays.
Samples in one batch were prepared and applied to the arrays, followed by detection of the proteins bound to the arrays with SELDI-TOF MS, on the same day.
Half of the case samples and half of the control samples were prepared and applied to the array on day 1 and the other half of the case and control samples were prepared and applied to the array on day 2. Detection of the proteins bound to the arrays was performed with SELDI-TOF MS for all arrays on the same day.
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After hybridization and washing, the DNA fragments bound to the array were eluted, using 300 μl of the elution buffer (Qiagen, Valencia, CA, USA) on each array.
To assay for cohesion, these cells contained a LacO array integrated at a CEN-proximal locus (TRP1), and a GFP-LacI fusion protein that bound to the array.
The absorber array is bound to the thermistor array in a collective process.
The scattering of sound by a tube array leads to two types of acoustic modes: those which are bound to the tube array and decay in the adjacent ducting and those which exist primarily in the ducting and tend to decay within the bundle.
The motion of these large regions in living mammalian cells was measured by monitoring the movement of the GFP-LacI signal bound to the LacO array at specific chromatin regions.
Additionally, small organic molecules known to bind to amyloid fibrils are commonly used to detect their presence: Congo red is particularly useful in this role as upon excitation with plane-polarised light the dye exhibits apple-green birefringence when bound to the regular array of β-strands within the cross-β structure of amyloid [40] (Fig. 2c, d).
The single-stranded PCR product was hybridized to the Sentrix Array Matrix where the labeled strand bound to the bead on the array containing the complementary address sequence.
Capture antibodies, specific for each biomarker are bound to the biochip surface defining arrays of test sites.
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