Sentence examples for bound to the affinity from inspiring English sources

When the activated ER bound to the affinity region, the molecular lost its swingable function, the electron transfer from the photo-excited dye to the SCE was consequently suppressed.

Proteins bound to the affinity matrix were packed to empty 10 ml columns (MoBiTec) and washed three times with MBP-buffer.

The proteins bound to the affinity columns were then eluted by addition of 50 mM and 1 M NaCl, and then 10 mM glutathione.

However, Ni-NTA affinity chromatography indicated that rDGAT1 from urea-solubilized samples bound to the affinity beads poorly because much less of the full-length protein was obtained in the urea-solubilized protein solution.

Significant amounts of MBP bound to the affinity resin and was purified to near homogeneity by the affinity chromatography, although lots of the recombinant MBP did not bind to the same affinity beads.

In order to recover all proteins bound to the affinity column, after passing protein extracts from C6/36 cells through DENV-2-Sepharose 4B column, the proteins were eluted from independent columns with buffer E containing 1 M NaCl, or 0.1 M Glycine pH 2.7.

The control dataset was generated by performing several pull downs in cells that expressed the vector alone to identify proteins that non-specifically bind to the affinity beads used in the purification.

Higher concentrations of competitor result in lower analyte retention times since the competitor also binds to the affinity column.

ArtinM is known to activate mast cells either by binding IgE bound to the high affinity IgE receptor (FcεRI) or by binding directly to FcεRI [3].

IgE bound to the high affinity IgE-receptor, FcεRI, on immune effector cells can travel to sites of allergen contact like mucous membranes and cause allergic symptoms.

Mast cells can be activated by cross linking of IgEs bound to the high affinity IgE receptor on their surface by antigen such as allergens [28].