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Results: In vitro studies: All three carriers preferentially bound to the activated rather than to quiescent HSC.
The originally-oxidized proteins are bound to the activated beads and recovered, washed and eluted in DTT-containing sample buffer for immunoblot analysis.
Light-scattering particles of iron oxide provided conspicuous contrast when bound to the activated endothelium under conditions of shear stress that were designed to mimic in vivo binding.
Then the MAb, diluted (0.1 mg/mL) in coupling buffer (10 mM sodium acetate, pH 4.5), was injected and bound to the activated carboxymethylated dextran surface via its primary amine groups.
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The murine cytomegalovirus (MCMV) induces in infected cells expression of the virus-encoded GPI-anchored m157 protein, which binds to the activating, DAP12-associating killer receptor Ly49H in MCMV-resistant mice leading to NKcell-mediated cytotoxicity.
Cell surface NKG2D ligands (NKG2DL) bind to the activating NKG2D receptor present on NK cells and subsets of T cells, thus playing a role in initiating an immune response.
Here we report that LZAP bound to the stress activated protein kinase p38, altered p38 subcellular localization and inhibited p38 phosphorylation and activity.
Cytochrome c and ATP are bound to the apoptotic protease-activating factor-1 [34].
Thus, although our preferred model of GxTx-1E bound to the activated/open Kv1.2/Kv2.1 paddle chimera represents the toxin bound to a lower affinity activated state of the Kv channel, it nicely illustrates how GxTx-1E could interact with the S3b helix in ways that closely resemble what is seen in the X-ray structure of PcTx1 bound to ASIC.
After complement proteins initially bind to the microbe, they activate their protease activity, which in turn activates other complement proteases, and so on.
These KDEL proteins can then bind to the KDELR, and activate the signalling cascade and the SFKs [ 10].
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