And, if the offending content is not cut, leading to a more forbidding rating, it becomes a "key classification issue", which the board is now bound to publicly specify.
For each sequence of interest, the two probes are joined by the use of a ligase only if they are both bound to a specified target sequence.
RIG-I (1 μM) bound to the specified RNA hairpin (1 μM) in buffer A (see above) was mixed with varying concentrations of mant-nucleotide (10 160 μM) under pseudo-first order conditions (>4× [RIG-I]) to measure the observed association rate constant.
Our previous study has demonstrated that 1 and 2 bound to the specified DNA fragment of the human breast cancer suppressor gene 1 (BRCA1) through the intercalative mode into the base pairs of DNA, and the DNA-binding constants (Kb) for 1 and 2 were 7.09 × 10 M-1 and 5.19 × 10 M-1, respectively.
These molecular devices can be used to rapidly characterize proteins and can be programmed to bind to specified regions of a protein.
They are: Calpers is legally bound to pay a specified level of benefits.
A variety of affinity tags have been developed, which strongly bind to a specified ligand [4], [5].
Many electors, however, are not legally bound to vote for a specified candidate.
Transfected cells were lysed with NETN buffer [50 mM Tris HCl at pH 7.5, 150 mM NaCl, 1 mM ethylenediaminetetraacetic acid (EDTA), 1% Nonidet P-40] and whole-cell lysates obtained by centrifugation were incubated with 10 μg of specified antibody bound to protein G Dynabeads (Invitrogen, Paisley, UK) for 2 hr at 4°C.
These privileges included the right to buy peasant serfs for labour in workshops, with the result that a class of "enlisted" serfs came into existence, living in specified areas and bound to the factories.
StudentsFirst's campaign expenditures are bound to grow next year, but Melton said it is too soon for him to specify which elections the group will play in.
