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Our results show that BLA exhibits a molten globule conformation when bound to negatively charged membranes.
Studies on polystyrene NPs demonstrated that proteins with isoelectric points (PI) of less than 5.5 like albumin adsorbed on positively charged particles whereas proteins with isoelectric points of higher than 5.5 like IgG bound to negatively charged particles.
The favorable changes in electrostatics in the E46K variant when bound to negatively charged lipids slow the dissociation of αS from the particle.
Aluminum is strongly bound to negatively charged groups in cell walls and is water-insoluble, hence it is not solubilized from the fiber fraction.
These two mineral elements are present at relatively high concentrations in the cytoplasm [ 17], and as the straw matures, they may become loosely bound to negatively charged components within the straw matrix.
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Chitosan could then bind to negatively charged bacterial surface, facilitating its antibacterial activity.
We show that BLA preferentially binds to negatively charged membranes at acidic pH with higher binding affinity.
The peptide also binds to negatively charged POPC/POPG (3:1) lipid vesicles but fails to insert deep into the membrane interior.
Despite high sequence diversity between the different genera, most parvoviruses bind to negatively charged glycans, such as sialic acid and heparan sulfate, abundant on cell surface membranes.
HPVs and numerous other sexually transmitted viruses initially bind to negatively charged cell-surface glycosaminoglycans (GAGs), particularly heparan sulfate proteoglycans (HSPGs), on cultured epithelial cells (reviewed in [21]).
The anticoagulant Annexin V preferentially binds to negatively charged phospholipids such as phosphatidylserine [ 12].
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