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Anti-HE4 biobodies were secreted directly biotinylated thus bound to labeled-streptavidin and streptavidin-coated surfaces without Ni-purification.
Consistent with this, no specific proteins bound to a labeled probe with A at SNP-358 (4th to 6th lanes).
In accordance with this hypothesis, both PPARα and PPARγ specifically bound to the labeled oligomer that corresponded to the -2150 promoter region (Fig. 7B, C).
Binding to the A probe was specific, as neither Foxp1 nor FOXP3-ΔN bound to a labeled oligonucleotide from the variable 1 region of the immunoglobulin promoter (V1P), which contains a canonical FOX consensus sequence defined for the HNF3/FOXA proteins (data not shown) [33].
FcεRI in the plasma membrane of mast cells were bound to fluorescently labeled IgEs, which were bivalent for 2,4-dinitrophenyl (DNP).
After rapid rinses with HEPES-buffered Hanks solution (HBHS), a 30 min incubation in 0.2 % Triton-X-100 (Sigma) in HBHS and a 30 min Image-iT FX signal-enhancer (Invitrogen) blocking step, astrocytes bound to catheters were labeled with 1 1000 monoclonal rat anti-glial fibrillary acidic protein (GFAP, Invitrogen) during incubations for 24 h at room temperature.
If all of the labeled probe bound to the cells, the maximum labeled probe on a cell was 2.8 femtomoles.
The red lines on detection pad were generated when the gold nanoparticles conjugated antibody bound to the fluorescein labelled amplicons, and the capture agents bound to their specific hapten on the other 5′ end of the double-stranded amplicon.
The biosensor consists of two labeled biomolecule architectures: a PRRSV-specific capture antibody labeled with a fluorescent dye bound to a protein that is labeled with a quantum dot and a PRRSV-specific capture antibody labeled with a fluorescent dye bound to a protein that is labeled with a gold nanoparticle.
Only the regulatory subunit bound to calcium ions chain labeled as chain D in this structure was used in the modeling.
This example could represent "static" mixture of free ligand molecules and static complexes (e.g. free labeled DNA and labeled DNA bound to an unlabeled protein as in the case of a binding experiment where the diffusion coefficient does not change significantly).
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