Sentence examples for bound to its operator from inspiring English sources

In the absence of Tc, TetR is tightly bound to its operator DNA; upon binding of Tc with an associated Mg2+ ion, it dissociates from the DNA, allowing expression of the repressed genes.

We demonstrate by deletion analysis that the extension contributes to DNA binding, and describe the crystal structure of SimR bound to its operator sequence, revealing that the N-terminal extension binds in the minor groove.

Alternatively it may be that occupancy of the promoter by just one or other of the repressors bound to its operator site might just reduce the chance of promoter activity rather than complete inhibition.

In that system, a transcription factor, the TetR protein from Escherichia coli, can bind to its operator sequence depending on the presence or absence of tetracycline or derivative compounds such as anhydrotetracycline or doxycycline.

One of its striking features is that even if the repressor (TetR2 protein) is bound to the operator tetO2, RNAp can still be recruited to the promoter tetP R 2 and start transcription if tetO1 is free [ 32].

Transcription of the fructose operon is repressed when Cra is bound to the operator, which is located on the downstream region of the RNA polymerase binding site [ 24].

The status of sister chromatid cohesion was analyzed by visualizing tetracycline repressor-GFP fusion proteins bound to tetracycline operator arrays integrated at the URA3 locus on chromosome 5, as previously described (Michaelis et al. 1997).

Presence of Tc causes TetR or Tet-OFF dimmers bound to an operator to unbind faster [ 35], meaning that that the complexes have a smaller half-life (2 min) as compared to the normal half-life of approximately 20 min. Similar to the binding of two Tc molecules to free TetR, the reaction of two Tc molecules with bound to an operator TetR is again broken down to two steps.

The inability of λcI to effectively act as a repressor when bound to a single operator at the downstream position is most probably caused by its inability to effectively compete with RNA polymerase binding at this position [13].

For example, suppose that a repressor tetramer is bound

Subsequently, Tc binds to the repressor (TetR2 bound to the operators or free) with high affinity.