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The presented method is expected to provide physiological relevant information because it closely simulates a physiological situation with the ligand bound to its native protein in the present of biological membranes.
present a model of the receptor CRF1R bound to its native ligand based on partial structures and 44 spatial constraints revealed by new crosslinking approaches.
A detailed molecular description of the interaction of VIP with one of its receptors, VPAC1 receptor, has recently been provided by performing extensive photoaffinity studies (Tan et al., 2003, 2004, 2006; Ceraudo et al., 2008a) in which the full-length ligand is covalently bound to its native receptor.
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DC-mediated but not direct administration of AdhPrP elicited antibodies that bound to murine native PrPc.
Several of the antibodies identified to recognize linear epitopes bound to native Crf2 on cells.
The cryo-EM structures presented in this investigation are of intact SARS spike bound to native virion lipid envelopes.
The scFv clones which bound to native Crf2 on A. fumigatus were further characterized by titration ELISA on biotinylated Crf2 coated on streptavidin plates (Fig. 7A).
These findings demonstrate that LA is bound to native HNF4α in vivo, and that binding is a physiological marker of the fed, but not the fasted, state.
The affinity of the scFvs which bound to native Crf2 and all scFvs derived from panning in solution was determined by surface plasmon resonance.
(C ) Staining of ferric iron (bound to ferritin) on native PAGE.
We observed that G3BP1 bound to native MK-STYX; however, binding to the mutant catalytically active form of MK-STYX was dramatically reduced.
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