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Presumably, these antisense DNAs bound to complementary sequences within the targeted spo0A mRNA and inhibited the translation of spo0A, thereby resulting in depression of spore formation in B. subtilis penetrons.
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When one or more input strands bind to complementary sequences on the loop, the loop breaks, activating the DNAzyme and switching the gate on.
However, they can themselves (epi-miRNAs) repress key enzymes that drive epigenetic remodeling and also bind to complementary sequences in gene promoters, recruiting specific protein complexes that modulate chromatin structure and gene expression.
Usually 25 bases in length, they bind to complementary sequences of RNA by standard nucleic acid base-pairing.
These non-coding ∼21 nt long molecules bind to complementary sequences on protein-coding messenger RNAs and as a consequence direct their degradation and/or repress their translation [12], [13].
Mostly, they function as translational repressors, which sequence-specifically bind to complementary sequences in the 3′-untranslational 3′-untranslationaleiregionet mRNAs.
MicroRNAs are post-transcriptional regulators that bind to complementary sequences in the 3'UTR of target mRNAs, usually resulting in mRNA degradation and gene silencing [25].
In E. coli, RyhB binds to complementary sequences of target mRNAs, causing their degradation in an RNaseE- and Hfq-dependent manner [23], [24], [25].
MiRNAs are a species of small regulatory RNA that bind to complementary sequences in mRNA and decrease gene expression by increasing degradation and blocking translation (reviewed in [26]).
miRNAs are post-transcriptional regulators that bind to complementary sequences in the 3′ UTRs of mRNAs.
They are normally 22 nucleotide RNA that binds to complementary sequences in the 3′UTR to repress gene activities [ 49].
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