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Acidic hydrolysis represents an effective method for the release of bound phenolic compounds trapped in the cores or the fruits and bound to cell wall matrix [ 28].
Furthermore, peroxidase zymograms of transgenic clones showed higher activity of a band with pI 9.6 in the fraction of ionically bound to cell wall proteins.
The group of cell surface-exposed proteins contains, besides membrane proteins with extended extracellular loops, also lipid-modified proteins, proteins covalently coupled to peptidoglycan by sortase, and cell wall-associated proteins not covalently bound to cell wall components [28].
Peroxidases are also involved in the biosynthesis of lignins, in the deposition of lignin bound to cell wall glycoproteins, and in the process of suberization [ 22].
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However, the possibility that some of the benzaldehyde units quantified by nitrobenzene oxidation could come from hydroxycinnamic acids bound to cell walls, because they share the aromatic skeleton with monolignols, cannot be ruled out.
Another method to assess cell wall composition is to measure the intensity with which antibodies that recognize specific epitopes within cell wall polymers bind to cell wall samples.
Two components are presumably bound to cell-wall components, i.e. the CscA is membrane-anchored and CscD is bound to peptidoglycan.
Interestingly, taking a broad view of the phylogeny of these three novel environmental isolates demonstrates that VHH B11 broadly binds to cell-wall proteins found in unicellular Chlorophycean algae (Additional file 1: Figures S1 and S2).
AgNPs can also directly penetrate into cells since they may bind to cell wall proteins that contain sulfur and phosphorus-containing biomolecules such as DNA [90, 91].
The high INA was mainly localized in the bark where it was tightly bound to the cell wall fraction (cell walls and/or intercellular structural components) while intracellular fractions had little or no INA.
After incubation at 100 °C for 10 min, the supernatant, corresponding to His7-MapZextra2 bound to the cell wall, was recovered from the cell wall pellet (pellet fraction P) by centrifugation (5 min at 5,000 × g).
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