However, nearly half of the fragments we isolated did not contain a canonical DBE or DBE-like sequence, suggesting that the DAF-16 DBD bound to a previously unknown binding element.
This is practice, Gloucester: By the law of arms thou wast not bound to answer An unknown opposite; thou art not vanquish'd, But cozen'd and beguiled.
However, the results are consistent with these two fragments not forming a complex which would suggest that PfRh1-240 may bind to an unknown membrane-bound protein allowing it to be escorted to the rhoptry neck where this family of proteins is initially located.
However, we do not exclude the role of HIF1α in Vegf induction during luteinization, because HIF1α may regulate Vegf expression at different time points of luteinization process, or bind to an unknown promoter or enhancer region of Vegf gene undergoing luteinization in GCs.
"We were fairly certain it was XPB because other researchers had found triptolide to bind to an unknown protein of the same size, but they weren't able to identify it," Liu said.
Binding to an unknown target can be influenced by cell absorption and transport, additional protein binding, secondary target interactions, drug metabolism, etc.
Interestingly an overlapping region of L2, residues 13 31, has been shown to bind to an unknown receptor on the surface of SiHa, CaSki, and HeLa cervical epithelial cells [33].
B7-H3 binds to an unknown receptor expressed on activated CD4+ and CD8+ T cells.
Because the mH2A antibody binds to an unknown epitope in the GV, immunofluorescence of transplanted nuclei was not possible.
