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After 30 months of passaging, we PCR amplified the 16 subtelomeric regions including TLOs from the populations of 18 independently evolved lines, using a chromosome arm-specific primer anchored in centromere-proximal, unique sequences together with a single, pan- TLO primer that bound to a sequence immediately downstream of the start codon and that is conserved among all TLO homologs.
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This ligand-bound VDR complex then binds to a sequence or sequences, called VDR response elements (VDREs), in the promoter region of target genes and acts as a transcription factor to promote gene expression.
NFAT transcription factors bind to a sequence in the IL-2 enhancer known as the antigen receptor response element 2 (ARRE-2).
Ii forms a homotrimer (Ii3) [1]–[3], and MHCII glycoproteins bind to a sequence of Ii that occupies the peptide binding groove of the αβ heterodimer [4] [6].
ABI4 is a nuclear transcription factor that binds to a sequence adjacent to or overlapping the G-box motif.
Clones that hybridized to a Psg32-specific oligonucleotide, Psg32-exon 1, which binds to a sequence internal to the PCR primers were sequenced.
A recent crystal structure of the Ffh protein bound to a signal sequence revealed a putative induced-fit mechanism that leads to accommodation of the signal sequence within the hydrophobic groove of the SRP protein [ 68].
One is based on the detection of SYBR Green bound to PCR products and the second method is more specific, detecting the cleavage of a fluorogenic (TaqMan) probe bound to a target sequence during primer extension phase.
The cell-permeable peptide Smac-N7 (AVPIAQK-PRQIKIWFQNRRMKWKK) bound to an antenopedia sequence facilitating incorporation into the cells was from Calbiochem.
pSTAT1 is a transcription factor that binds to a consensus sequence termed Gamma Activation Sequence or GAS (TTC n2-4 GAA) [44].
The system includes a DNA-cutting enzyme, Cas9, bound to a short RNA guide strand that is programmed to bind to a specific genome sequence, telling Cas9 where to make its cut.
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