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The RNA stem loop is bound to a conserved binding groove in the catalytic domain.
Here, Janus et al. report the crystal structure of the antigen-binding fragment of a pan-reactive antibody bound to a conserved epitope of the glycoprotein, facilitating rational design of cross-protective vaccines and therapeutics.
Mutagenesis and a reconstruction by negative-stain electron microscopy of the Fab in complex with trimer revealed that it bound to a conserved epitope, which stretched across gp120 and gp41.
Complexes with HAs from the group 1 H1 and the group 2 H3 subtypes analyzed by x-ray crystallography showed that the antibody bound to a conserved epitope in the F subdomain.
It bound to a conserved epitope in the stem similar to CR6261 and F10, using different approach by avoiding contact with nearby glycan in Group 2 viruses which extended its breadth to both groups.
X-ray crystal structures of Head modules from Saccharomyces cerevisiae and Schizosaccharomyces pombe have been determined, with (Robinson et al., 2012) and without (Imasaki et al., 2011; Lariviere et al., 2012) the carboxy-terminal domain (CTD) of the largest pol II subunit, Rpb1, bound to a conserved site on the surface.
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By studying how the patients' antibodies react to H5N1 influenza, Theraclone scientists found that the most effective antibodies bound to a spot that appears conserved among all viral strains, a specific location on a known surface protein called M2.
These primer pairs specifically bind to a region conserved in all wPip groups for each gene (Supplementary Table 2).
GATA type transcription factors are zinc finger containing proteins that bind to a highly conserved DNA sequence motif ('WGATAR'WGATAR
E2C is a homodimeric β-barrel protein (36− 41), which binds to a highly conserved pseudopalindromic target site.
They bind to a highly conserved region in the HA stem region and share the fusion peptide as a part of their epitopes.
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