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DNA microarray is an array of oligonucleotide probes bound to a chip surface [ 231, 232].
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In principle, one of the proteins was bound to a sensor chip, and the compounds were allowed to pass over the surface.
Similar results were obtained from both the covalently immobilized A3 and the Bt-A3 bound to a NeutrAvidin coated chip, with both yielding a KD of ~ 400 to 600 pM.
This was proven by Jung et al., who quantified biotin-functionalized vesicles bound to a streptavidin-coated chip from the SPR signal based on the mass of lipids in the vesicles using a method described elsewhere and showed that the SPR signal was proportional to the number of bound vesicles per unit area.
The purified sbAvd-1 and sbAvd-2 bound to a testosterone-BSA-coated sensor chip with similar affinities (Table 1), whereas wtAvd showed no binding to the testosterone surface.
To confirm the protein identities obtained from sequencing, a SELDI immunoadsorption approach was performed using an ApoC-I antibody bound to an RS100 protein chip array.
Fusions of VEGF-R2 or VEGR-R3 with the crystallizable fragment (Fc) of human IgG (VEGF-R2-Fc or VEGF-R3-Fc) were bound to an anti-Fc coated BIAcore chip to generate a homogenous receptor surface.
The latter thus corresponds to an MBP-Chip dimer bound to an SSDP tetramer (expected 181 kDa), the only possible stoichiometry that fits the observed molecular mass.
Yeah, they would get switched from study hall to P.E in a flash, where they are bound to chip a nail.
Fiedler, Graeb, Mieszczanek et al. discovered that Pygo directly binds to a protein complex called Chip/LDB-SSDP (or ChiLS for short).
We compared the binding curves for each antibody format using three different densities of NIP10-BSA covalently bound to the chip surface.
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