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Furthermore, a yeast TPR domain co-chaperone, Sti1 known to interact with the C terminus of Hsp90, bound simultaneously with Sgt1 (Catlett and Kaplan, 2006).
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In all cases the two acetylated lysines bound simultaneously and with identical conformations to the BRD4(1) Kac binding site.
The nearly complete loss of binding to R9G/H10Y and S104E indicates that both regions are bound simultaneously, consistent with the distances between the amino acids and the observed maximum dimensions of single epitopes of up to 21 × 28 Å.
The four deadenylases (CNOT6, CNOT6L, CNOT7 and CNOT8) are not bound simultaneously and associate with the complex in a mutually exclusive fashion, whereas CNOT4 associates only transiently with the CCR4-NOT complex4.
We have previously identified R-thiomandelate as a promising reasonably broad-spectrum inhibitor of MBLs, combining a thiol functionality which binds simultaneously to the two zinc atoms with a carboxylate function which increases its inhibitory potency [ 21, 27].
One potential mechanism for ensuring pairing-interaction specificity is the use of sequence-specific DNA-binding proteins that can bind simultaneously with two or more recognition sequences.
Prp17 binds simultaneously with Cwc2 indicating that it is part of the core NTC complex.
Tsr1 could therefore bind simultaneously with the H27 28 and H44 45 regions.
Or, any or all of Shc (pY1148), Shc (pY1173), and Grb2 (pY1068) can bind simultaneously with Stat5 (pY992).
In the usual description of the mechanisms of scaffolding, scaffold proteins are considered as reactors where molecules involved in a cascade of reactions are simultaneously bound with the right orientation to meet and interact.
Because the number of gene copies is restricted and only a limited number of regulators are able to bind simultaneously, the kinetic law saturates both with the amount of activator and inhibitor.
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