Sentence examples for bound scFv were detected from inspiring English sources

Bound scFv were detected as previously described.

Bound scFv were detected as described above using the murine 9E10 monoclonal antibody detecting the MYC-tag.

Bound scFv were detected using murine mAb 9E10 which recognises the C-terminal c-myc tag and a goat anti-mouse serum conjugated with horseradish peroxidase (HRP) (Sigma; 1 10,000).

Bound scFv were detected using mouse α-c-Myc-tag 9E10 (culture supernatant, 100 μL, 1 1,000 in PBST-B; 1.5 h at RT) followed by goat α-mouse IgG (Fc -HRP (A0168, Sigma-Aldrich, Germany) (100 μL, 1:30,000 in PBST-B; 45 min at RT).

Then, scFv was added at constant concentration and bound scFv was detected by anti-penta-His antibody.

In the first format, serial dilutions of HER-2/neu ECD-Fc fusion protein, ranging in concentration from 25 to 0  μg ml−1, were used as inhibitor solutions; bound soluble scFv was detected as previously described.

The ELISA was performed as described above except that after incubation of E. coli HB2151 supernatants, bound soluble scFv was detected using HRP-conjugated anti-FLAG-tag M2 Ab (Sigma) for 1.5 h.

Cells were washed with PBS and bound phage scFvs were detected using ABTS (Sigma Chemicals, Saint Louis, MO, USA) as a substrate.

Periplasmic extracts were applied and after washing with PBS, bound scFv was eluted with 100 mM triethylamine and neutralised with 1 M Tris, pH 7.4.

Without IPTG induction, no scFv was detected even in a whole cell fraction.

Bound scFv phage were detected by using the mAb anti-M13 conjugated with horseradish peroxidase (HRP) (GE Healthcare, München, Germany; 1 5.000).