Sentence examples for bound proteins were washed from inspiring English sources

The bound proteins were washed by buffer and eluted by 7 mM glutathione.

Bound proteins were washed and reserved with the unbound fraction for Western blot analysis.

The resins with bound proteins were washed thoroughly and finally the proteins were eluted with 10 mm maltose in elution buffer (50 mM PIPES, pH 6.8, 100 mM NaCl, 1 mM EGTA and 0.2 mM MgCl2).

Bound proteins were washed and subjected to SDS-PAGE.

Beads containing bound proteins were washed four times with TAP IP buffer followed by elution with 2× SDS PAGE buffer.

Bound proteins were washed with lysis buffer and competitively eluted using lysis buffer supplemented with 250 µg/mL FLAG peptide.

The MagneHIS beads with bound protein were washed three times with wash buffer.

The beads with bound protein, were washed three times with PBS plus protease inhibitors (2 µg/ml pepstatin A, 2 µg/ml leupeptin, 1 mM phenyl methyl sulphonyl fluoride, 1 mM benzamidine) and 1 mM DTT.

Ni-bound proteins were washed (PBS, 500 mM NaCl, 20 mM imidazole) and eluted for 30 min at room temperature (PBS, 500 mM NaCl, 200 mM imidazole).

Resin-bound proteins were washed three times with 30 mM HEPES, 150 mM NaCl, 20 mM imidazole, pH 7.4, followed by batch elution with 30 mM HEPES, 150 mM NaCl, 300 mM imidazole, pH 7.4.

Bound protein was washed, and suspended in sample buffer.