Sentence examples for bound proteins were released from inspiring English sources
After washing the column with B-PER wash buffer, the bound proteins were released with elution buffer (50 mM Tris, 300 mM NaCl, 200 mM imidazole, 10% [v/v] glycerol), according to a previous protocol (Shibasaki et al. [2013]).
Bound proteins were released by incubating with Laemmli sample buffer.
Bound proteins were released from the beads by boiling in SDS sample buffer and separated on a 4 12% Tris-Glycine gel in MES buffer.
Bound proteins were released by washing with 50 µl of 25 mM Tris-HCl, 100 mM NaCl, 2 mM 2-mercaptoethanol, 500 mM imidazole (pH 8.0).
Bound proteins were released from the antibody-coated beads using 200 mM glycine, pH 3.0.
Bound proteins were released by addition of SDS-sample buffer and separated by SDS-PAGE.
The DNA and the bound proteins are released from the beads by cleaving with a restriction enzyme specific for a recognition sequence included into the bait oligo sequence.
Soluble and insoluble proteins are recovered following treatment with 0.1% Triton X-100, and chromatin bound proteins are released by inclusion of 0.5 M or 2.0 m NaCl or subsequent treatment with DNase I. NM bound proteins are defined as those which are not released by any of these treatments.
Matrix-bound proteins were released by boiling in denaturing buffer and detected by SDS-PAGE with Coomassie blue staining.
Matrix-bound proteins were released by boiling in denaturing buffer and detected by SDS-PAGE with Coomassie blue staining (panel 1) or by western blotting to confirm the identities of the bound Gag (panel 2, anti-MA and anti-CA) and NEDD4L (panel 3, anti-NEDD4L) proteins and help to distinguish background proteins from low-level binding in panel 1.
Matrix-bound proteins were released by boiling in denaturing buffer and detected by SDS-PAGE with Coomassie blue staining (panel 1) or by western blotting (panel 2, anti-NEDD4L) to confirm the identities of the bound NEDD4L and help to distinguish background proteins from low-level binding in panel 1.
