Traditionally the term "interaction strength" refers to how tightly two proteins bind physically.
Numerous other pioneer proteins bind physically to PtdIns(4,5 P2, similar to AP-2 (Höning et al., 2005; Jackson et al., 2010), and some contain EH and SH3 domains, producing a densely populated interactome to coordinate membrane remodeling, coat biogenesis and cargo selection.
The drug is either covalently bound or physically entrapped in polymer matrix, depending on the method of preparation [ 23].
Type XII and XIV collagen can be extracted without proteolysis, so they appear not to be covalently polymerized in the matrix (Watt et al., 1992), but are thought to bind physically to collagen fibril surfaces via their COL1/NC1 domains.
In addition, Ctf4 was shown to bind physically to the catalytic subunit of polymerase α [14].
However, whether sclerostin and Lrp4 bind physically to each other, or whether they form an indirect functional interaction has not been known.
PACSIN/Syndapins are EFC (extended FCH (Fes/CIP4 homology)) domain proteins implicated in endocytosis because the C-terminal SH3 domain binds physically to the large GTPase dynamin, the phosphoinositide polyphosphatase synaptojanin, or the branched-actin regulator WASp [18] [20].
Finally, to provide evidence linking the sex-related locus to sexual reproduction will require, for example, documentation that the genes therein are expressed at an appropriate time in the life cycle, or to show a candidate protein binds physically to the promoters for meiotic gene homologs by chromatin immunoprecipitation studies.
Second, in GST pull-down assays, the minimal FCHO1 linker (residues 316 467) binds physically to cytosolic AP-2.
This heterotetrameric complex binds physically to the FCHO1, FCHO2 and Sgip1 linker regions, and the presence of the bound AP-2 subunit chains is confirmed by immunoblotting.
