Sentence examples for bound phages were detected from inspiring English sources

After incubation for 2 hours at 37°C unbound phages were washed off and bound phages were detected using horseradish peroxidase-conjugated anti-M13 antibody (Amersham Pharmacia Biotech Inc., Piscataway, NJ, USA) diluted in blocking buffer.

The bound phages were detected by adding 50 μl HRP-conjugated anti-M13 antibody (Pharmacia, diluted 5000 times with PBS) followed by an incubation of 1 h.

The bound phages were detected as described above using HRP-conjugated anti-M13 polyclonal antibody.

Bound phages were detected using rabbit anti-mouse IgG antibody conjugated with horseradish peroxidase (HRP).

Bound phages were detected using an in-house produced guinea pig-anti-phage polyclonal antiserum (1 : 1000) and HRP-labelled swine anti-guinea pig antiswine anti-guineaako, Copighantiserummark).

Bound phages were detected with horseradish peroxidase (HRP -conjugated anti-M13 mAb (GE HRP -conjugatedcataway, NJ, USA), scFv with an HRP-labelled anti-FLAG mAbmAb (SiGE Healthcareand scFv-Fcε fusion Piscatawayith aNJHRP-labelled anti-IgE antiserUSA(KPL, Guildford, UK) uscFv 1-Step Ultra TMB – ELISA Substrate (Pierce, Rockford, IL, USA) as chromogen.

After 10 cycles of washing with 1x TBS, bound phages were eluted using 0.2 M glycine, pH 2.2.

Specific bound phages were eluted, amplified and used for next panning as previously described [ 14].

Specific bound phages were eluted, amplified and used for next round of selection as described [ 12].

The pre-cleared phages were incubated with HEK293-CD9 cells, and bound phages were recovered and amplified for next round of panning.

Remaining bound phage were eluted and allowed to infect E. coli cells.