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Four h post administration, cells were washed extensively with PBS and non-internalized bound particles were removed by incubating the cells with 0.05% trypsin-EDTA (Gibco) and 0.4 mg/ml protease K (Qiagen) until cells were detached from each other and from Transwell filters.
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Non-specifically or weakly bound phage particles were removed by washing ten-times with 0.1% TBST.
IHD-J F13 GFP EVs were bound to HeLa cells on ice and incubated either on ice or at 37°C for a further 30 min. Bound virus particles were removed by trypsin digestion and the cells were subjected to flow cytometry to determine the cell-associated fluorescence from internalized particles.
On Nov. 27, Ward underwent arthroscopic surgery in which loose particles were removed from his knee.
Undissolved matrix particles were removed by centrifugation.
Unbound particles were removed by washing.
Unbound particles were removed with further washes with PBS-T.
Hairs and other non-dust particles were removed manually.
Smaller particles are removed via the upper-side outlets and larger particles are removed via the bottom outlet.
Soluble particles are removed into the bloodstream and are eventually excreted by the kidneys.
The aggregation of these particles is removed and a well separated individual particle has been formed.
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CEO of Professional Science Editing for Scientists @ prosciediting.com