Brx1 and Rpf2 form an extensive interface with their binding partners Ebp2 and Rsr1, but Rpf1 and Imp4 primarily bind only a single α-helix of Mak16 and Mpp10.
However, since ABPs are also inhibitors of their target enzymes, they bind only a single target protease molecule and fluorescent signals are not amplified by multiple processing events.
These derivatives bind only a single copper atom, suggesting that they indeed correspond to enzyme states with each of the two copper sites singly occupied.
An emulsion is then created that most of the beads bind only a single DNA molecule followed by the second round PCR.
S. cerevisiae kinetochores bind only a single microtubule, at least during mitosis, and coordination between multiple microtubule binding sites is required only during the first meiotic division when sister kinetochores must behave as a single unit and attach to microtubules from the same pole.
The cavities within pseudo wild-type and L121A lysozymes each bind only a single atom of noble gas, while the cavities within mutants L133A and F153A have two independent binding sites, and the L99A cavity has three interacting sites.
However, antibodies bind only to a single epitope, and mutations that confer resistance to antibody-mediated inhibition of HIV entry have been detected.
Alternatively, do linear motifs that bind only in a single orientation do so because of spatial constraints inherent to their cellular functions?
Although quite elegant, these models envision the stator as fixed during ATP synthesis, i.e., bound to only a single αβ pair.
The generation of Nrf2 mutants that bind with either lower or higher affinity to Keap1 than wild-type (WT) Nrf2 showed that if the low affinity 'DLG' motif in Nrf2 is mutated, so that Nrf2 is bound to only a single member of the Keap1 dimer, the E-FRET signal at 21% is lost, whereas the E-FRET signal at 13% is maintained.
