Sentence examples for bound material was washed from inspiring English sources
Bound material was washed with 20 mM sodium phosphate buffer (pH 7.2) and then eluted with 0.2 M acetic acid, pH 2.7, and lyophilized.
Bound material was washed with wash buffer (10 mM Tris-HCl pH 8, 250 mM NaCl, 0.1% NP-40, 1 mM EDTA, 1 mM DTT, 5% glycerol) and elution was achieved by boiling the beads in Leammli buffer.
The bound material was washed using 2 × 1 mL of a 3% (v/v) aqueous MeCN followed by 1 mL of a 10% (v/v) aqueous MeCN, and the elution was conducted by the application of 2 × 500 µL of an 80% (v/v) aqueous MeCN.
Unbound material was washed out, then bound material was separated by SDS-PAGE and visualized with Coomassie blue.
After hybridization, non-specifically bound material was removed by washing and specifically bound target was detected using the GeneChip Hybridization, Wash and Stain kit, and the GeneChip Fluidics Station 450 (Affymetrix).
Subsequently to hybridization, non-specifically bound material was removed by washing and detection of specifically bound target was performed using the GeneChip® Hybridization, Wash and Stain kit, and the GeneChip® Fluidics Station 450 (Affymetrix).
Following hybridization, non-specifically bound material was removed by washing and detection of specifically bound target was performed using the GeneChip® Hybridization, Wash and Stain kit, and the GeneChip® Fluidics Station 450 (Affymetrix).
Beads were washed and bound material was analysed by Western blot as described previously (Boëda et al., 2007).
Unbound material was washed off with running buffer, and bound material was eluted with a two-stage linear gradient of high-salt buffer (buffer B) [10 mM Na2HPO4 and 1 M NaCl (pH 7.4)] at a flow rate of 1 mL/min, and 1 mL fractions were collected.
