After hybridization, non-specifically bound material was removed by washing and specifically bound target was detected using the GeneChip Hybridization, Wash and Stain kit, and the GeneChip Fluidics Station 450 (Affymetrix).
Subsequently to hybridization, non-specifically bound material was removed by washing and detection of specifically bound target was performed using the GeneChip® Hybridization, Wash and Stain kit, and the GeneChip® Fluidics Station 450 (Affymetrix).
Following hybridization, non-specifically bound material was removed by washing and detection of specifically bound target was performed using the GeneChip® Hybridization, Wash and Stain kit, and the GeneChip® Fluidics Station 450 (Affymetrix).
Following hybridisation, non-specific bound material was removed by washing with the GeneChip Hybridisation, Wash and Stain kit (Affymetrix, CA, USA) and the GeneChip Fluidics Station 450 (Affymetrix, CA, USA).
Unbound material was removed by three washes with lysis buffer J. Bound material was removed from the beads by incubating beads in 250 mM imidazole (Sigma Aldrich Co., I0125) for 5 min on ice.
Non-bound material was removed by washing the beads four times with BN-lysis buffer, and bound material was eluted by competition with synthetic myc peptide (Sigma) for 2 h at 4 °C.
Material was removed in 76% of the cases.
