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Input corresponds to 25% of the starting material; 50% of the bound material was loaded per lane; no binding was observed when CaM was omitted from the beads (data not shown).
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The beads were washed three times with 150 μL of binding buffer, and bound material was eluted by boiling in SDS loading buffer.
The beads were washed four times with binding buffer, and bound material was eluted by denaturation in SDS loading buffer and detected by western blotting with anti-FLAG antibody.
Unbound material was washed out, then bound material was separated by SDS-PAGE and visualized with Coomassie blue.
There, contaminated material was loaded into steel tanks prior to being loaded onto ships.
Material corresponding to the total and to 1/10th of the material was loaded for eosinophils and γδT cells respectively.
After extensive washing, bound materials were eluted with Laemmli buffer and analysed by immunoblotting.
After preliminary sorting at Pier 96, for example, many of San Francisco's salvaged materials are loaded straight into containers bound for China, where they will be combed over much more thoroughly before being recycled.The biggest American exporter by volume is a firm called American Chung Nam.
The starting materials were loaded into liquid ammonia solution and stirred for 6 hours.
A ship bound for Turkey is loaded with scrap metal.
Sometimes, their cytoplasm was loaded with phagocytized material).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com