Sentence examples for bound material was eluted from inspiring English sources

Beads were pelleted for 30 sec at 2000 g in a microcentrifuge and bound material was eluted with 25 µl of 2x SDS sample buffer, heated for 5 min, and frozen at −20°C.

Specifically bound material was eluted with 300 mM NaCl, 500 mM imidazole, 100 mM Tris (pH 7.4) and submitted to non-reducing SDS-PAGE.

The beads were washed three times with 150 μL of binding buffer, and bound material was eluted by boiling in SDS loading buffer.

After extensive washes, bound material was eluted using RNase H (New England Biolabs (NEB), UK) for 30 min at room temperature.

After another wash with 40 μl of 5 mM glucose 6-phosphate (Sigma Aldrich), the specifically bound material was eluted with 40 μl of 5 mM M6P (Sigma Aldrich) in the binding buffer.

Beads were washed again and bound material was eluted from beads with 50  μl SDS-PAGE sample buffer at 50 °C, using microSpin columns (Promega, San Luis Obispo, CA, USA) at 5000 × g for 5 min.

The eluate was allowed to flow through the cartridge by gravity, the column was washed with deionised water and bound material was finally eluted with 2 ml 60% acetonitrile in deionised water and lyophilised in a Speed-Vac (Heto Lab Equipment, Allerod, Denmark).

The bound materials were eluted by a buffer containing FLAG peptide (0.2 mg/ml).

Finally, the remaining material was eluted with 1 wt% DOC, which resulted in complete elution.

Unbound material was eluted with 15 ml of PBS and 1 ml fractions were collected.

Imidazole (10 mM) was applied to the beads to wash away nonspecifically bound materials, and XLF was eluted with 100 mM imidazole.