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The bound material was detected using the biotinylated antibodies followed by streptavidin conjugated with horseradish peroxidase.
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After hybridization, non-specifically bound material was removed by washing and specifically bound target was detected using the GeneChip Hybridization, Wash and Stain kit, and the GeneChip Fluidics Station 450 (Affymetrix).
The beads were washed four times with binding buffer, and bound material was eluted by denaturation in SDS loading buffer and detected by western blotting with anti-FLAG antibody.
Unbound material was washed out, then bound material was separated by SDS-PAGE and visualized with Coomassie blue.
The material was detected Wednesday, meaning it might have leaked a day or two after the earthquake, Tokyo Electric said.
Biotinylated material was detected by dansyl, FXIIIA and TG2 antibodies.
No embolization material was detected in the lung.
In immunofluorescence studies however, nearly no filamentous material was detected.
Immunoreactive material was detected by the enhanced chemiluminescence technique (Amersham).
If dangerous material were detected at any time, the information would be relayed to authorities by wireless communication technology.
Flake later issued a statement on Wednesday saying no dangerous materials were detected in the mailings.
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