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After an overnight incubation at room temperature, the resin was washed three times in pulldown buffer containing 20 mM imidazole, and twice more in 8 M urea, 100 mM sodium phosphate, pH 8.0, 10 mM Tris HCl and 30 mM imidazole, before bound material was analysed by gel electrophoresis and western blot.
Beads were washed and bound material was analysed by Western blot as described previously (Boëda et al., 2007).
Bound material was analysed by western blotting with relevant antibodies.
Similar(57)
Unbound material was washed out, then bound material was separated by SDS-PAGE and visualized with Coomassie blue.
The available statistical material was analysed.
The material was analysed in a qualitative content analysis process.
Twenty-two osteosarcell celinesnes derived from patient material were analysed.
After extensive washing, bound materials were eluted with Laemmli buffer and analysed by immunoblotting.
From standard curves of 5% or 10% reference material, 1% reference materials were analysed.
The material obtained was analysed using the TMS method [ 55- 57].
Behaviour was analysed using the video material.
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