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Biotinylated proteins and bound mAbs were visualized using a horseradish peroxidase complex or peroxidase-conjugated secondary antibodies (Dako, Hamburg, Germany) and chemiluminescence (ECL, Amersham Biosciences, Chalfont St. Giles, UK).
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The biotinylated mAbs were visualized by Alexa Fluor 568-conjugated streptavidin (Invitrogen, Carlsbad, CA) and were observed with a confocal microscope MRC-10244, Bio-Rad, HerCAles, CA).
After a final wash, bound antibodies were visualized by SuperSignal West Pico chemiluminescent substrate kit (Pierce).
Following further washing, bound antibodies were visualized as above.
Subsequently, bound antibodies were visualized using the anti-rabbit Envision Plus System (K4011, Dako, Glostrup, Denmark).
Binding profiles were visualized with the Integrated Genome Browser (IGB) browser [87].
Binding antibodies were visualized by using ECL and ECL Plus kits (GE Healthcare).
Binding antibodies were visualized by ECL plus chemiluminescence system (GE Healthcare).
Antibodies bound to the plate were visualized with NBT/BCIP reagents (Thermo Fisher Scientific).
The vegetative cells were incubated with the mAb, and were visualized with FITC-conjugated goat anti-mouse IgG (green).
The specific binds were visualized by enhanced chemiluminescence reagents and recorded on film.
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