Sentence examples for bound mAbs were detected from inspiring English sources
Bound mAbs were detected by incubation with AP-conjugated goat anti-human IgG (Southern Biotech).
Bound mAbs were detected with biotinylated anti-mouse IgG, extravidin-peroxidase, and enhanced chemiluminescence.
Bound MAbs were detected by immersing the membrane in TTBS-1% (w/v) gelatin containing 1/3000 dilution of goat anti-mouse-IgG antibody conjugated to alkaline phosphatase (GAM-AP, Bio-Rad) for 1 hour whereas bound PAbs were detected by using goat anti-rabbit-IgG antibody conjugated to alkaline phosphatase (GAR-AP, Bio-Rad).
Bound Mabs were detected either by incubation with 100 μl HRP-conjugated polyclonal anti-mouse IgG diluted 1 1,000 in PBS/Tween (non-biotin-labelled Mabs) or by incubation with 100 μl StrepABComplex diluted 1 50 (DAKO Cytomation, Glostrup, Denmark) with biotin-labelled Mabs for 1 h at 37°C and developed with ABTS (2,2'-azino-di-3-ethylbenzthiazoline-6-sulfonic 2,2'-azino-di-3-ethylbenzthiazoline-6-sulfonic 2,2'-azino-di-3-ethylbenzthiazoline-6-sulfonic 2,2'-azino-di-3-ethylbenzthiazoline-6-sulfonic 2,2'-azino-di-3-ethylbenzthiazoline-6-sulfonic 2,2'-azino-di-3-ethylbenzthiazoline-6-sulfonic 2,2'-azino-di-3-ethylbenzthiazoline-6-sulfonic
Sections were washed and bound mAb was detected by incubation with AlexaFluor568-conjugated goat anti-mouse IgG (Invitrogen) for 1.5 hours diluted in RPMI/10% FCS.
Unconjugated mAbs were detected with anti-mouse IgG-POD (1∶5000; Sigma) as secondary antibody.
Binding of all these unlabelled mAbs was detected by phycoerythrin (PE -conjugated secondary reagents to IgG (Sigma–Aldrich) or total mouse Ig (Dako).
By contrast, only negligible levels of mAb were detected in control mice administered A2C9-1+PBS.
After incubation for 1 h, plates were washed and bound biotinylated mAb was detected using AP-labeled streptavidin (Jackson Immunoresearch).
In some individuals, a dull staining with the F4 mAb was detected.
After six washes, bound Ab were detected with biotinylated anti-mouse IgG1 (BD8-2, BD) or anti-mouse (5.7, BD) mAb or with biotin-SP-conjugated anti-mouse IgG F ab′ 2 (Jackson Immunoresearch).
