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The paper deals with the application of Volterra bound Interval type −2 fuzzy logic techniques in power quality assessment.
With this scheme it is possible to divide the problem into subproblems, solving the classification of the web service requests in a more simple and effective way and always within a time bound interval.
We found 1,376 HLH-1 bound peaks genome wide (Table S1) with bound interval sequences averaging 1,454 bp in length.
In order to assay the potential biological relevance of the HLH-1 binding sites identified by ChIP, we generated GFP reporter genes by placing HLH-1 bound interval sequences upstream of a myo-2 gfp myo-2 gfpominimal
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We did not anticipate the large number of HLH-1 bound intervals and associated genes, up to half of all protein coding genes in C. elegans in the ChIP-seq data using default threshold values.
The distribution of HLH-1 bound intervals relative to the TSS revealed the −200 to −400 bp region as the most frequent location of binding sites (Figure 1) and identified 1,032, of the predicted ∼20,000 (WS195) protein encoding genes, as potential targets of HLH-1 regulation (Table S2).
These results suggested that the vast majority of HLH-1 bound intervals detected in embryonic chromatin by array hybridizations are factor-specific and are reproducibly detected by deep sequencing or qPCR, with the confidence of detection directly related to the peak score.
It was possible that the failure of HLH-1 bound intervals to enhance bodywall muscle reporter gene expression in wild type animals was due to the relatively low levels of endogenous HLH-1 compared to the levels of heat shock induced HLH-1 or epitope-tagged HLH-1 used to identify potential binding sites by ChIP.
We assigned both the preferentially bound intervals and the qualitatively uniquely bound intervals to target genes.
Additional file 9: Target genes annotated to conserved, commonly bound intervals.
Here we have found a clear preferential conservation of commonly bound intervals between D. melanogaster and D. simulans.
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