When a crystal of IPK was soaked in a stabilizing solution containing IP, Mg2+, and ATPγS, the product IPPβS was observed bound in the active site.
The zinc-bound structure has two metal ions bound in the active site in all protomers.
In the absence of a peptide-bound crystal structure, a model of vCPH was generated with peptide PKPAPK bound in the active site (see Experimental Procedures).
We suggest that such a conformational change likely occurs only after both substrates are simultaneously bound in the active site.
That is, L-arginine is so tightly bound in the active site that any destabilization of the highly specific interactions increases the overall degrees of freedom.
We report the crystal structure of AKR1C1 complexed with the first structure-based designed inhibitor 3-chloro-5-phenylsalicylic acid (Ki = 0.86 nM) bound in the active site.
A peptide bound in the active site mimics a phosphotyrosine substrate, affords insight into substrate recognition, and provides a testable substrate prediction.
Each protomer can be subdivided into three domains and the prosthetic group, FAD is bound in the active site between domain I and domain II.
Herein, we report the high-resolution X-ray crystal structures of the DapE from Haemophilus influenzae with one and two zinc ions bound in the active site, respectively.
For different families of chitinolytic enzymes, the number, type, and position of substrate atoms bound in the active site vary, resulting in different substrate-binding specificities.
