Similar to the H2T assay (Fig. 1a, left panel), the H3T assay (center panel) utilizes a pair of monoclonal antibodies that bind epitopes in the C-terminal, intracellular region of the receptor.
Ezrin is synthesized in a dormant form in which the N-terminal domain in bound to the C-terminal domain, thus mutually blocking their capacity to bind other molecules [ 10].
In the ADP bound structure, the C-terminal tail and Gly-rich loop are more closed than in the open state adopted in the apo structure but are also much more open than the intermediate or closed conformations.
As another case example, the crystal structure of neurotensin receptor NTSR1 has been reported in an activelike conformation bound to the C-terminal portion of the agonist NTS.
The results support a mechanism where cleavage of the tyrosine radical occurs heterolytically to form a 4-oxidobenzyl radical and dehydroglycine, in which dehydroglycine is bound to the C-terminal cluster.
PARP-1 bound to the C-terminal 289 390 aa of NEIL1 in the in vitro binding assay.
Further insights into diatomic ligand formation have been provided by Britt and co-workers, who have reported EPR signals at g ≈ 9.5 and 5, proposed to arise from a [3Fe 4S]+ cluster bound to the C-terminal cluster site, in S. oneidensis HydG.
Homology modeling of PfHop and HsHop in complex with their own cytosolic Hsp90 and Hsp70 C-terminal peptide partners indicated excellent conservation of the Hop concave TPR sites bound to the C-terminal motifs of partner proteins.
The first two PDZ domains of PSD-95 in turn bind to the C-terminal tail of stargazin, a transmembrane regulatory subunit of AMPA (α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid -receptors thacid -receptorsfor AMPA-recepthattraffisking and gating (criticalal., 2000; Tomita et al., 2005; Nicoll et al., 2006; Yokoi et al., 2012).
Using just the EF-hand, Wharton et al., found this region was sufficient to bind Dvl [10], while Yan et al., found Dvl still bound Nkd in the absence of the EF-hand [22] and we previously found that Dvl binds a domain in the C-terminal region on Nkd2 [20].
