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The chemical -beta- [125I]iodo-4-hydroxyphenyl -ethyl- aminomethyl-tetralone bound in a specific, saturable manner to a single class of binding sites in membranes that expressed recombinant hamster alpha-1B, bovine alpha-1C and rat alpha-1D receptors expressed in rat-1 fibroblasts and to those from prostate tissue.
Crucially, this revealed that each chemical bound in a specific manner with different stereochemistry by hydrogen bonding to different residues located within the antibody binding site.
Material has been rendered radioactive with 125I and between 20 and 25% of the radioactivity is bound in a specific manner to the antiserum.
In addition to confirming the observed binding preferences, the structural data also visualize that both Hsp70 and Hsp90 chaperones are bound in a specific manner and are thus likely candidates to assist UNC-45 in myosin folding and assembly.
A high affinity binding site has been characterized using radioligands in U937 monocyte membranes, where a novel radiolabelled EET agonist bound in a specific, saturable and reversible manner, resulting in the production of cAMP production with similar potency as 11,12-EET 11,12-EET-EET.
During a titration experiment, Ffree is obtained from eq 1b and the fraction of DNA bound in a specific complex is described by 3cand Fbound-NS can be calculated according to These values were substituted into eq 3b to yield an expression that describes ⟨ s*⟩ as a function of [IHF].
Similar(54)
The NMR-determined structure of the 1 DNA complex verified that the molecule bound in a sequence-specific fashion with the linkers residing in the minor major minor grooves as predicted.
Transcriptional activators and repressors bind in a sequence-specific manner to promoters or enhancers of target genes.
An attractive alternative to FISH is chromosome labeling using synthetic polyamide probes, which bind in a sequence-specific manner but do not require denaturation of DNA.
Plasma membranes from P-gp-expressing cells bound [3H]tamoxifen in a specific and saturable fashion.
However, recent evidence suggests that VEGF can also bind in an isoform-specific fashion to a newly identified neuropilin-1 (NP-1) receptor.
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