Sentence examples for bound fractions were released from inspiring English sources

Bound fractions were released by heating at 70°C for 10 minutes in 20 μl NuPAGE LDS sample buffer.

5% input and bound fractions were analyzed by immunoblotting with anti-GFP and anti-HA antibodies.

Input and bound fractions were treated with RNase A and proteinase K (both from Roche).

5% input and bound fractions were analyzed by immunoblotting with anti-GFP antibody (upper panel).

The input, unbound supernatant, and bound fraction were resolved on a gel and subjected to autoradiography.

Further, the magnetic field is removed and bound fraction is eluted from the column.

The glycosidically bound fraction was composed of ketones, alcohols, terpenes and norisoprenoids.

If diluted to 90% only a small fraction is released (15%).

Our results thus suggest that the Zn deposited by atmospheric pollution mainly remains loosely bound and is released in the 0.1 M HCl leach fraction, giving rise to the heavier isotopic signature observed in the acid leachate (Table  3).

In this assay, loosely bound histone is released at lower salt concentrations than tightly bound proteins.

This is because all the chemically bound water is released before phase A can be stable.