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Flow was directed over a mock surface to which no protein was bound, followed by the HA proteins coupled surface.
Anti-SOCS6 serum was affinity-purified by incubation with nitrocellulose membrane strips to which the same recombinant protein was bound, followed by elution with 0.2 M glycine (pH 2.7) and immediate neutralization using 1 M Tris (pH 8.0).
At that point, a new FPP molecule binds, while the farnesylated protein remains bound, followed by product dissociation either prior to or concerted with binding of a new CaaX-box substrate protein.
MD simulations of conformations of the Mhp1 protein with L-BH or L-IMH bound followed our previous work (Shimamura et al, 2010) with some differences as described in Supplementary Methods.
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Theresa was determined: wherever her beloved went, she was bound follow.
Hence the lower bound follows from Lemma 3.1.
Here, the (C^{1,1}) bound follows directly from Assumption C. (iii) (d>0, w in Gamma ^0).
Here, the (C^{1,1}) bound follows directly from Assumption C. (d>0, w in Gamma ^0).
end{aligned}The (L^{infty }) bound follows from the equivalence of the norms in the space (mathcal {P}_2).
The O bound follows from the fact that (left ({n atop k}right geq left (frac {n}{k}right)^{k}).
In this study, the power-law aperture distribution with a lower bound follows the form of Eq. (1), in which α should be larger than 1.
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