After addition of internal standards, the "free" and "bound" lipid extracts were methylated and silylated and subsequently analyzed by GC and GC-mass spectrometry (GC-MS).
Neither the T C nor the C-to-A mutated ERE fragments at the indicated sites (see Materials and Methods), bound nuclear extracts of the colon cancer cell line studied.
The cream of the collection is now on exhibit through May and is bound to extract a few wows even from people who couldn't tell a T square from a protractor.
But Peter Smedvig, who moved to London in 1991 for tax reasons and runs a $250 million private equity fund, was duty bound to extract as high a price as possible for his birthright.
This made it of interest to characterize whether TRP1 and TRP2 bound nuclear extract proteins and if so which promoter elements were critical for this binding.
Method validation was done by redocking the bound ligand extracted from the active site of hCA-II (3K34).
All available structures of proteins expressed in E. coli with either ATP or an ATP analogue bound were extracted from the Protein Data Bank (PDB).
Mass spectrometry of proteins extracted from EMSAs of TRP1 and TRP2 bound by nuclear extracts of T. gondii were then performed.
The cell wall anchored IsdB protein is recognized as the predominant Hb receptor, and is comprised of two NEAr transporter (NEAT) domains that act in concert to bind, extract, and transfer heme from Hb to downstream Isd proteins.
Supershift assays using specific antibodies revealed that normal nuclear extracts bound c-Jun and, to a lesser extent, JunD.
