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Thus, the presence of a second ISWI monomer bound did not affect the rate at which the nucleosome was repositioned, suggesting that a monomeric ISWI is sufficient to obtain the observed repositioning activity.
These values were used as additional constraints in a global analysis of all repositioning time courses where we found that the presence of a second ISWI monomer bound did not affect the rate at which the nucleosome was repositioned, suggesting that a monomer is sufficient for the observed repositioning activity.
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Surprisingly, Rab3a locked in the GDP- or GTP-bound conformation did not affect the number of docked LDCVs, indicating that the active conformation is not sufficient to promote docking (arrows in Fig.1G, H; Fig 1N).
As shown in Figure 5C, the presence of GTPγS-bound or GDPβS-bound His-Gα16 did not affect the ability of GST-Fhit to hydrolyze Ap3A.
W-5 alone did not affect the amount of CaM-bound htt-exon1-44Q, but once again the amount of CaM-bound htt-exon1-44Q was significantly lower when 10 µM of CaM-peptide was present.
Finally, pretreatment of RA ECs with an excess of the CXCR4-specific inhibitor T134, which precludes CXCL12α binding to CXCR4 [ 20], did not affect the immunodetection of RA EC-bound CXCL12.
There were no significant differences in the amounts of cell-bound Aβ42 between control and squalestatin-treated neurons, indicating that cholesterol depletion did not affect the binding of Aβ42 to neurons.
The more conservative G342A mutation did not affect the level of binding to ANTXR2, but ANTXR2-bound PA-G342A prepores exhibited a pH threshold higher than that of wild-type prepores.
Indeed, recently, has been proposed that ER-bound transcripts escape interactions with SGs during stress [14]; in yeast, mutations that prevented SG formation did not affect the stabilization of mRNAs, which also imply the existence of alternative factors [33].
This did not affect the outcome.
PP did not affect the G6PD activity.
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