Sentence examples for bound complexes were washed from inspiring English sources
Bound complexes were washed three times with lysis buffer and once with TBS/0.05% Tween 20.
The bound complexes were washed and collected by centrifugation and separated by SDS-PAGE.
The bound complexes were washed twice in Low Salt Solution, twice in High Salt Solution, once in LiCl and once in Tris/EDTA (TE) buffer.
Bound complexes were washed six times with RIPA (50 mM HEPES pH7.6/1 mM EDTA/0.7%DOC/1%Igepal/0.5.5 M LiCl) and TBS and then eluted from the beads with elution buffer (50 mM NaHCO3/1% SDS).
Bead-bound complexes were washed four times with cold lysis buffer then boiled in Laemmli sample buffer and loaded onto an SDS-PAGE apparatus.
Bead-bound complexes were washed four times in lysis buffer, boiled in Laemmli sample buffer and analyzed by western blotting after SDS-PAGE fractionation.
After four washes in wash buffer, bead-bound complexes were washed twice in wash buffer containing 0.5 M NaCl and twice in kinase assay buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 25 mM KCl, 5 mM MgCl2, 0.02% Triton X-100).
Bead-bound complexes were washed four times in wash buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 5 mM EDTA, 5% glycerol, and 0.1% Triton X-100), and bead-bound proteins were eluted by boiling in 80 µl of sample buffer, and examined by SDS-PAGE and Western blot.
Antibody-protein G Sepharose bound protein complexes were washed three times with IP buffer (25 mM Tris-HCl pH 7.9, 10% (v/v) glycerol, 0.1% NP40, 0.5 mM DTT, 5 mM MgCl2) containing 0.5 M KCl and twice with IP buffer containing 100 mM KCl.
The beads bound with immune complexes were washed to remove nonspecific binding and resuspended in 250 μl of digestion buffer containing proteinase K. Finally, the MeDIP DNA was purified with phenol/chloroform extracting and then ethanol precipitated.
