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Bound complexes were visualized using DAB (3,3'-diaminobenzidine) reagent, counterstained with haematoxylin, dehydrated, and mounted with Permount.
Bound complexes were visualized using diaminobenzidine reagent (Sigma-Aldrich, St . Louis MO, USA), counterstained with hematoxylin (Merck & Co., Inc., Whitehouse Station, NJ, USA), dehydrated, and mounted with Permount (Fischer Scientific, New Jersey, USA).
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Images of ligand and receptor bound complexes were prepared in Ligplot visualizing program [ 31] and polar contacts between them were noted down.
The structures for the inhibitor bound complexes were retrieved from Protein Data Bank with PDB codes: 1AJX [32] for AHA001 bound complex, 1HVR [33] for XK263 bound complex and 1HXW [34] for ABT538 bound complex.
Bound antibody complexes were visualized with 3,3'-diaminobenzidine (DAB, Sigma-Aldrich).
Protein-DNA complexes were visualized by phosphoimager.
Immune complexes were visualized by enhanced chemiluminescence (PerkinElmer Life Sciences).
These complexes were visualized with tapping mode Atomic Force Microscopy (AFM).
Antigen-antibody complexes were visualized using Immobilon Western reagent (Millipore).
The hPS-CT 4 complexes were visualized with PyMOL [38].
Gels were dried and protein-DNA complexes were visualized using Fuji PhosphorImager.
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