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Bound complexes were separated on 6% polyacrylamide gels, dried and visualized on X-ray film.
Bound complexes were separated on 8% polyacrylamide gels, blotted onto membrane, and visualized by autoradiography (Hyperfilm, Fuji).
Immediately following the incubation, the bound complexes were separated from the free probe by electrophoresis on 4% nondenaturing polyacrylamide gels in 0.5× Tris-borate-EDTA buffer for 40 min at 4°C.
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After extensive washing with CB300, bound complexes were eluted with FLAG-peptide and separated by SDS-PAGE.
The structures for the inhibitor bound complexes were retrieved from Protein Data Bank with PDB codes: 1AJX [32] for AHA001 bound complex, 1HVR [33] for XK263 bound complex and 1HXW [34] for ABT538 bound complex.
Bound protein complexes were separated on SDS/12.5% PAGE gels.
DNA-bound protein complexes were separated by electrophoresis.
MAR complexes were separated by blue native electrophoresis.
The complexes were separated using the EMSA.
The protein complexes were separated over night at 4°C.
Individual proteasome complexes were separated using native gel electrophoresis.
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